Analysis the Key Enzymes Producing Arginine from Corynebacterium crenatum

Abstract

Abstract: The key enzymes producing arginine from Corynebavterium crenatum were investigated by the methods of affinity and SDS-PAGE. The results indicated that one of the key enzymes was acetylglutamate kinase with around 34 kD. estimated by SDS-PAGE, and the other protein with around 49kD. estimated by SDS-PAGE, showed the affinity to arginine. The above mentioned results indicated that the key enzymes, restrained by arginine in arginine metabolize path of Corynebacterium crenatum, were not the only one. The properties of acetylglutamate kinase were also primarily studied. The results indicated that the optimum reaction temperature was 49℃ and optimum pH 8.0. The protease activity of the enzyme was strongly inhibited by Pb2+、Cu2+、Mn2+ and Fe2+, and was affected definitely by DTT.

Key words: Corynebavterium crenatum, arginine, key enzyme

CHEN Xue-Lan, XU Zheng-Hong, TAO Wen-Yi. Analysis the Key Enzymes Producing Arginine from Corynebacterium crenatum[J]. FOOD SCIENCE, 2005, 26(3): 35-39.

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Analysis the Key Enzymes Producing Arginine from Corynebacterium crenatum

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