FOOD SCIENCE ›› 2005, Vol. 26 ›› Issue (5): 194-197.

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Study on Determination of Trifolium pratense Isoflavones by Three-wavelength UV Spectrophotometry

 CHEN  Han-Qing, JIN  Zheng-Yu   

  1. School of Food Science and Technology, Southern Yangtze University
  • Online:2005-05-15 Published:2011-09-19

Abstract: Three-wavelength UV spectrophotometry was established to determine the content of total isoflavones in Trifolium pratense. This method could eliminate interference of pigments and ethanol-soluble proteins in the ethanol extract of Trifolium pratense. The relative standard deviation (RSD) of precision test was 0.85%, the average recovery of adding sample test was 103. 95% and RSD was 1.82%. It was a simple, fast and accurate method for determination of Trifolium pratense isoflavones.

Key words: Trifolium pratense isoflavones, determination, Three-wavelength UV spectrophotometry