FOOD SCIENCE ›› 2019, Vol. 40 ›› Issue (16): 261-266.doi: 10.7506/spkx1002-6630-20180926-282

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Simultaneous Determination of Free Gossypol and Its Degradation Product Tetramethoxy Gossypol in Commercially Available Cottonseed Oil by High Performance Liquid Chromatography

ZHOU Mengyu, Gulisitan·ABULAITI, YAO Jun, CHEN Ling   

  1. College of Pharmacy, Xinjiang Medical University, ürümqi 830011, China
  • Online:2019-08-25 Published:2019-08-26

Abstract: This study aimed to establish a high performance liquid chromatography (HPLC) method for the determination of free gossypol and its degradation product tetramethoxy gossypol in commercially available cottonseed oil in order to provide a scientific basis for quality control of cottonseed oil. The chromatographic separation was accomplished on an Inertsil? ODS-3 column (250 mm × 4.6 mm, 5 mm) using a mobile phase comprised of methanol and 2% phosphoric acid (85:15, V/V) at a flow rate of 1.0 mL/min, and the column temperature was set at 32 ℃. The detection wavelength was 235 nm, and the injection volume was 10 mL. Good linear relationship was achieved for gossypol in the concentration range of 0.245–1.226 mg/mL (r = 0.999 5). The relative standard deviation (RSD) of precision and repeatability was 0.88% and 1.73% (n = 6), respectively. The average recovery was 101.2%, with RSD of 1.75% (n = 3). The method also showed a good linear relationship for tetramethoxy gossypol in the concentration range of 0.211–1.056 mg/mL (r = 0.999 6). The RSD of precision and repeatability was 1.56% and 1.11% (n = 6), respectively. The average recovery was 100.3%, with RSD of 1.12% (n = 3). Free gossypol was detected in 25 out of 90 batches of commercially available cottonseed oils in the range of 0.159– 1.661 mg/mL, and tetramethoxy gossypol in 56 in the range of 0.093–1.661 mg/mL. This method displayed good accuracy, high precision and easy operation and could be widely used to monitor the detoxification of cottonseed oil.

Key words: high performance liquid chromatography, free gossypol, tetramethoxy gossypol, determination

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