FOOD SCIENCE ›› 2021, Vol. 42 ›› Issue (4): 287-296.doi: 10.7506/spkx1002-6630-20191008-045

• Safety Detection • Previous Articles     Next Articles

Rapid Screening of Pesticide Residues in Pepper Powder by High Performance Liquid Chromatography Coupled with Quadrupole-Time of Flight Mass Spectrometry

ZHU Xiaoling, LIU Jie, YU Tingting, ZHANG Li, WANG Huixia, WU Wanqin, CAO Qi, ZHANG Yazhen, YANG Zong   

  1. (1. Hubei Provincial Institute for Food Supervision and Test, Hubei Provincial Engineering and Technology Research Center for Food Quality and Safety Test, Wuhan 430070, China; 2. SCIEX, Shanghai 200335, China)
  • Online:2021-02-25 Published:2021-02-25

Abstract: An analytical method was established for the simultaneous determination of 169 pesticide residues in pepper powder by high performance liquid chromatography coupled with quadrupole-time of flight mass spectrometry (HPLC-Q-TOF MS). The samples were soaked in 0.1% (V/V) aqueous acetic acid solution, then extracted with acetonitrile and purified with C18 and Florisil sorbents. The compounds were separated on a C18 column (100 mm × 2.1 mm, 2.6 μm) using gradient elution with a mobile phase composed of 5 mmol/L ammonium formate aqueous solution and methanol, detected in the positive ion mode and quantitated by the matrix matched external standard method. Meanwhile, an accurate mass database and a fragment ion library were created for rapid qualitative and quantitative analysis of the 169 pesticides in pepper samples. The results showed that the method had a good linearity (R2 > 0.95) within the concentration range of 5–100 ng/mL for 136 pesticide compounds. The remaining 33 compounds could only be qualitatively analyzed. The recoveries for the target compounds at three spiked levels ranged from 26% to 140%, with relative standard deviations of 1.5%–36.0%. The method is simple, less time consuming and suitable for rapid and high-throughput screening of pesticide residues in pepper powder samples.

Key words: high performance liquid chromatography coupled with quadrupole-time of flight mass spectrometry; screening; pesticide residues; pepper powder

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