Isolation and Purification of Lactoferrin in Bovine Colostrums and Delection of Its Immune Function

Abstract

Abstract: The lactoferrin was purified by centrifugation and ammonium sulfate precipitation from the bovine colostrums. Thepurity of lactoferrin after CM-Sephadex C50 ion-exchange chromatograph and Heparin-Sepharose CL-6B affinity chromato-graph was 76.98%and 92.75% respectively. The two kinds of lactoferrin isomers were assayed by SDS-PAGE and HPLC withthe molecular weights 76000Da and 78000Da respectively. The purified sample and standard lactoferrin showed the sameimmunogens through immunoelectrophoresis. The isolated lactoferrin improved the immune function significantly in miceexperiments.

Key words: colostrums, lactoferrin, isolation and purification, immune function

YE Zhen-Min, WANG Zhi-Geng, YU Wei-Yi. Isolation and Purification of Lactoferrin in Bovine Colostrums and Delection of Its Immune Function[J]. FOOD SCIENCE, 2005, 26(7): 208-211.

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Isolation and Purification of Lactoferrin in Bovine Colostrums and Delection of Its Immune Function

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