FOOD SCIENCE ›› 2005, Vol. 26 ›› Issue (7): 122-124.

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Study on L-Glutamine Fermentation

 YANG  Yan-Ling   

  1. 1.Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fujian Agriculture and ForestryUniversity;2. Open Laboratory of College of Life Science, Fujian Agriculture andForestry University;3.College of Bioengineer, Hubei Industry University
  • Online:2005-07-15 Published:2011-09-19

Abstract: The L-glutamine fermentation was carried out with a SG resistant strain Carynebacterium Glutamicum S9114, andthe optimum conditions for producing glutamine were listed as follows: the carbon source was 18% hydrolysed starch and thenitrogen source was 4% NH4Cl, Zn2+ concentration was 20mg/L and CaCO3 concentration was 5%. The 500ml flask were filled30ml media with pH7.0 to incubate 72h, with inoculation amount 10%. Under optimum condions, Carynebacterium GlutamicumS9114 was able to accumulate the maximum quantity of 52.6mg/ml with the average of 49.5mg/ml of L-glutamine.

Key words: glutamine, fermentation, amino acids, SG-sulfaguanidine