Molecular Cloning, Sequence Analysis and Primary Expression of Mucor pusillus DNA

Abstract

Abstract: In this paper, rennin gene was amplified from Mucor pusillus and sequenced. The nucleic acid sequence and amino-acid sequence encoded by the gene were compared using DNAman biological software. Results showed that amplified fragmentwas a novel rennin gene(mcp). re-combination Pichia pastoris KM71/PIC9K-mcp was constructed successfully and obtained amulti-copied integrated strain of MK3 by resistant screening (G418). Initial fermation experiments were carried out andmethanol was used as sole carbon source. The enzyme activity of re-combination MK3 was 5.4U/ml in above condition.

Key words: rennin, clone, sequence analysis, re-combination strain, express

QIU Zhong-Yan, XU Min, WANG Zheng-Xiang. Molecular Cloning, Sequence Analysis and Primary Expression of Mucor pusillus DNA[J]. FOOD SCIENCE, 2005, 26(7): 58-63.

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Molecular Cloning, Sequence Analysis and Primary Expression of Mucor pusillus DNA

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