FOOD SCIENCE ›› 2007, Vol. 28 ›› Issue (10): 384-388.

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Study on Assay Conditions for Tea β-D-galactosidase Activity and Its Diversities in Various Tea Species, Season and Leaf Tenderness

 YU  Zhi, HUANG  Hai-Bo, ZHANG  Yun, NI  De-Jiang   

  1. Key Laboratory of Horticultural Plant Biology Ministry of Education, College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan 430070, China
  • Online:2007-10-15 Published:2011-11-22

Abstract: β-D-galactosidase was extracted from tea leaves. Single factor analysis and orthogonal experiment were adopted to obtain the optimal conditions for activity assay. The results showed that the activity ofβ-D-galactosidase can be greatest if it is investigated at 60 ℃ for 7 min with citrate-phosphate as buffer (pH4.0). The difference of β-D-galactosidase activities in tea species, seasons and tenderness were analyzed. It was found that there are obvious different activities between oolong tea and green tea species. At the same time, the activity diversity also exists in various seasons and leaf tenderness.

Key words: &beta, -D-galactosidase; activity; tea cultivar; season; tenderness;