Abstract: Glutathione is an important active substance in body, it exist in all of the biological cell.The key point obtaining glutathione from wheat germ is that making glutathione release from cell into solution . HEFP can break the cell membrane and improving its passing ability. Applying high electric field pulse (HEFP) to wheat germ is used in this study. The experimental result show that this treatment way obtaining glutathione from wheat germ by HEFP is feasible and the effect is good . In the other hand, The product of glutathione is affected by the intensity and pulse of electric field and the water-wheat germ rate,and pulse of electric field is main effects.The experimental result also show that optimum obtaining conditions are Intensity of Electric Field 16kV, Pulse of Electric Field 9,Water-wheat germ rate 1:20.

Key words: high electric field pulse(HEFP), wheat germ, glutathione