A Preliminary Study on the Separation of Carotene, Xanthophylls and Chlorophylls on Reversed-phase C18 Supercritical Carbon Dioxide Chromatography

Abstract

Abstract: A primary investigation into the separation of carotene, xanthophylls and chlorophylls on reversed-phase C18 supercritical carbon dioxide chromatography was made in this study. Carotene, xanthophylls and chlorophylls were readily separated under the conditions as follow: Stationary phase: Zorbax ODS; Mobile phase: 3% (V/V) propanol-1 in CO2; Supercitical conditions: pressure: 4000psi; temperature: 40℃; flow rate: 2.0ml/min; detection: 435nm. In comparison with the chromatographic profit of those fractions on reversed-phase C18 HPLC, their SFC profit showed a normal phase pattern. This observation suggested that supercritical carbon dioxide was strong apolar and pre-packed reversed phase C18 column could be employed as normal phase stationary phase for the separation of those compounds.

Key words: carotene, xanthophyll, chlorophyll, SFC

HUI Bo-Di. A Preliminary Study on the Separation of Carotene, Xanthophylls and Chlorophylls on Reversed-phase C18 Supercritical Carbon Dioxide Chromatography[J]. FOOD SCIENCE, 2005, 26(8): 162-166.

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A Preliminary Study on the Separation of Carotene, Xanthophylls and Chlorophylls on Reversed-phase C18 Supercritical Carbon Dioxide Chromatography

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