Abstract: Object: To study the submerged culture of Clitocybe maxima mycelia and its effect on skeletal muscle fatigue. Method: Selecting the optimum carbon sources, nitrogen sources and inorganic salt to get the optimum liquid medium recipe by the orthogonal test in three factors and four levers; Isolated toad gastrocnemius stimulated by pulse electricity was regarded as fatigue model and Clitocybe maxima mycelia was compared with Ringer solution to see what influence they had on muscle contract ability. Results: The optimum liquid medium recipe was brown sugar 2%, wheat-bran 3%, KH2PO4 0.2%. Clitocybe maxima mycelia of 0.8mg/ml and 0.3mg/ml could delay the time when muscle contract extent was 90%, 50% and 10% of its maximum, while that of 0.1mg/ml could delay the time when contract extent was 90% of its maximum(p<0.05). Conclusion: Clitocybe maxima mycelia had the function of delaying fatigue of isolated skeletal muscle.

Key words: Clitocybe maxima, submerged culture, orthogonal test, isolated gastrocnemius, muscle fatigue