FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (10): 81-85.doi: 10.7506/spkx1002-6630-201010016

• Processing Technology • Previous Articles     Next Articles

Ultrasonic Pretreatment Followed by Enzymatic Hydrolysis for Extraction of Polysaccharides from Fermented Mycelia of Phellinus linteus

XIE Li-yuan1,2，WANG Fu-wei3，LI Hong-jun1，GAN Bing-cheng2,*，PENG Wei-hong2，TAN Wei2，HUANG Zhong-qian2   

  1. 1. College of Food Science, Southwest University, Chongqing 400716, China；2. Institute of Soil and Fertilizer, Sichuan Academy
    of Agricultural Sciences, Chengdu 610066, China；3. College of Resources and Environment, Sichuan Agricultural University,
    Ya an 625014, China
  • Received:2009-07-30 Online:2010-05-15 Published:2010-12-29
  • Contact: GAN Bing-cheng

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Abstract:

To develop an optimal procedure based on ultrasonic pretreatment followed by enzymatic hydrolysis for the extraction of polysaccharides from the fermented mycelia of Phellinus linteus, single factor and orthogonal array design methods were used to deal with the effects of crucial ultrasonic pretreatment and hydrolysis parameters on polysaccharide yield. Results showed that the optimization of ultrasonic pretreatment led to 3.356% polysaccharide yield, in which, the optimal values of ultrasonic power, pretreatment time and material/liquid ratio were determined to be 500 W, 20 min and 1:25 (g/mL), respectively. The optimization of enzymatic hydrolysis leading to 6.619% polysaccharide yield was achieved through the combinatorial use of cellulase at 2.5% dosage, pectinase at 2.5% dosage and neutral proteinase at 1% dosage for the hydrolysis for 120 min at pH 6.5 and 50 ℃. The combination of ultrasonic pretreatment and enzymatic hydrolysis may provide a good idea for the large-scale production of polysaccharides from the fermented mycelia of Phellinus linteus.

Key words: Phellinus linteus, polysaccharide, ultrasonic, compound enzyme, orthogonal test

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