FOOD SCIENCE ›› 2021, Vol. 42 ›› Issue (7): 169-175.doi: 10.7506/spkx1002-6630-20200506-043

• Nutrition & Hygiene • Previous Articles     Next Articles

Preparation, Structural Characterization and Probiotics Proliferation-Promoting Activity of Selenized Dandelion Polysaccharide

WANG Libo, GAO Jingyu, LI Tengfei, LI Lianyu, LIU Bo, ZHANG Hongyan, YANG Yu, XU Yaqin   

  1. (College of Arts and Sciences, Northeast Agricultural University, Harbin 150030, China)
  • Online:2021-04-15 Published:2021-05-17

Abstract: In this study, a selenized polysaccharide from dandelion roots (Se-DRP) was prepared by HNO3-Na2SeO3 method. The physicochemical properties, structural characteristics, probiotics proliferation-promoting activity of Se-DRP and its inhibitory effect on α-amylase were investigated. The results showed that Se-DRP was a heteropolysaccharide with a molecular mass of 8 102 Da, and it was composed of galacturonic acid, rhamnose, arabinose, mannose, glucose and galactose with a molar ratio of 0.64:0.73:19.36:0.84:1.00:27.41. Nuclear magnetic resonance (NMR) analysis showed that the major sugar residues in Se-DRP were →6)-β-D-glucose-(1→, →5)-α-L-arabinose-(1→, →3)-β-D-galactose-(1→ and →4)-α-D-galacturonic acid-(1→. Se-DRP possessed probiotics proliferation-promoting activity and could be utilized by Lactobacillus plantarum 10665 and Lactobacillus acidophilus as a carbon source. In addition, Se-DRP exhibited α-amylase inhibitory activity with an inhibitory rate of (52.34 ± 1.45)% at a concentration of 4.0 mg/mL. These results provide a theoretical basis for further studies of Se-DRP and the utilization of dandelion resources.

Key words: selenized dandelion polysaccharide; structural characterization; physicochemical properties; prebiotic proliferation; α-amylase inhibitory activity

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