Abstract: The effects of solid media, hormone and light for inducement from Ginkgo biloba embryo to callus growth were studied. The effects of hormone and carbon for callus growth and flavone output were studied as well. The results showed that: (1) The explant cultured on DCR medium with 1.0 mg/L 2,4-D +0.5 mg/L BA in dark is of the optmum conditions to induce callus growth. (2) 0.3 mg/L BA+2.0 mg/L 2,4-D hormone not only accelerate callus growth but also heighten flavone contents in seccedent culture.(3) Carbon inhibitates callus growth, but enhances flavone accumulation.

Key words: culture condition, Ginkgo biloba, callus, flavone content