Abstract: Three species of algae were collected from the coast of Yantai. After being surface disinfected, endophytic fungi were isolated with PDA medium. The purified endophytic fungi were cultivated in liquid medium for seven days. After centrifuging, mycelia were dried and grinded, then extracted with 95% alcohol. The antifungal activity of the fementation broch and ethanol extracts of mycelia on seven kinds of plant pathogenic fungi were assayed by means of mycelium growth rate. The results showed that all of the fermentation broth exhibited more than 50% antifungal activity against three kinds of botanical pathogenic fungi, especially the antifungal rates of the fermentation broch of the endophytic fungi isolated from Undaria pinnatifida on six kinds of plant pathogenic fungi were more than 50% even to the highest of 78.8%, which meaned they can produce extracellular metabolites with wide spectrum and high efficiency. But the antifungal activity of mycelia extracts is not remarkable, which meaned the intracellular metabolites of the three endophytic fungi were inactive.

Key words: alga, endophytic fungi, antifungal activity