FOOD SCIENCE ›› 2005, Vol. 26 ›› Issue (9): 52-57.

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Study on the Separating by TLC and Properties Defining of the Pigments from Loropetalum chinense var.rubrum flower

 TANG  Ke-Hua, CHEN  Xuan, CHEN  Gong-Xi   

  1. 1.Hunan Key Laboratory for Forest Chemical Engineering; 2.Department of Agriculture and Foresty, Jishou University
  • Online:2005-09-15 Published:2011-09-20

Abstract: Seperating by silca-G&H thin-layer chromatography(TLC) on the pigments from Loropetalum chinense var.rubrum flower, the optimum volume ratio of the seperating layer reagent is water12 to eathnol 8 to plus-butanol 24 to acetic ester36. To developing the flower pigment by the optimum layer reagent, seven colour spot, which obtained from the pigment , have the same colour and the same Rf. Seperating and scanning results showed that the characteristic wavelength of the pigment in the L. chinense var.rubrum flower is 277nm and it,s seven mono-component are 272, 278, 277, 270, 273, 274, 271nm repectively, and the maximum content of the mono-component is the third component (277nm). Combinding the results of scanning on the pigment component with the chracteristic colour reaction in Anthocyanidin and Flavonoid and Anthraquinone of the pigment, the main mono-component in the flower pigment must belongs to flavanonol.

Key words: Loropetalum chinense var.rubrum, flower petal, pigment, thin-layer chromatography(TLC), separation, properties definition