Cloning and Sequence Analysis of N-methyltransferase in Camellia ptilophylla Chang

Abstract

Abstract: In this study, with complete coding sequences (CDS) of N-methyltransferase (NMT) genes, the tea varieties of Camellia.sinensis var. assamica.cv. yinghong 9, Camellia sinensis cv. Fuyun 6 and Camellia ptilophylla chang were cloned with RT-PCR, and the deduced amino acid sequences were 365aa, 369aa and 369aa respectively. The cDNA sequences of NMT were analysed and compared with other NMT. The NMT genes obtained from Camellia show above 86.5% homology in DNA sequence and above 86.3% in amino acid sequence, while all the cDNA include six conserve sequences, three SAM combined region of A, B’, C and one VFFF conserve domain. Based on sequence homology comparisons, the differences in DNA sequence of Camellia ptilophylla chang are presumed to have changed the NMT substrate specificity and led to different accumulation of purine alkaloids.

Key words: , tea； N-methyltransferase (NMT)； gene clone；

CHEN Li-Ping, CHEN Zhong-Zheng, LI Bin, ZHOU Ying, LI Qiu-Hua, YANG Fei-Yun. Cloning and Sequence Analysis of N-methyltransferase in Camellia ptilophylla Chang[J]. FOOD SCIENCE, 2006, 27(7): 99-103.

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Cloning and Sequence Analysis of N-methyltransferase in Camellia ptilophylla Chang

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