Identification of Food-borne Staphylococcus aureus by Microarray Technique

Abstract

Abstract: Objective: The goal of this study was to establish a method for identifying rapidly food-borne Staphylococcus aureus by microarray technology. Methods: The target gene of 16SrRNA form S.aureus was amplified by PCR (polymerase chain reaction) and the sequence was compared for BLAST(basic local alignment search tool) in genbank. The oligonucleotide hybridization probe was designed to identify samples. Results: The specimens were detected by microarray hybridization. The result of microarray hybridisation for food-borne Staphylococcus aureus showed that it is positive. Conclusion: The food-borne Staphylococcus aureus is identified rapidly and accurately with microarray hybridization technique so it is worth propagation.

Key words: Staphylococcus aureus, microarray, identification

LI Yu-Feng, HE Yang, LIU Hong-Lu. Identification of Food-borne Staphylococcus aureus by Microarray Technique[J]. FOOD SCIENCE, 2007, 28(12): 294-297.

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Identification of Food-borne Staphylococcus aureus by Microarray Technique

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