Abstract: Honey and milk powder could be used by lactobacillus to make honey fermentation sour milk which reserve the nutrition components and flavor of honey. Meanwhile, it was also a new path to exploit the honey. The experiment studied the activity of invertase, glucose oxidase and β-galactosidase and the influence of enzymatic activity on the reducing sugar conversion rate. We got the optimal conditions: the time is 4.0h, the honey was 8% and the inoculation was 3%. Accordingly, the activity of invertase was 23.845 mg/g·h; the activity of glucose oxidase was 10.621μg/g·0.5h and the activity ofβ-galactosidase was 1.211μg/μmol·min; the reducing sugar conversion rate was 44.34%. In this condition, we got the best product.

Key words: honey； lactic acid； invertase； glucose oxidase； &beta, -galactosidase；