FOOD SCIENCE ›› 2007, Vol. 28 ›› Issue (12): 327-331.

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Agrobacterium-mediated Transformation of Tomato with Anti-LeERF2 Gene

 LI  Ying-Cong, ZHU  Ben-Zhong, LUO  Yun-Bo   

  1. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China
  • Online:2007-12-15 Published:2011-11-22

Abstract: Agrobacterium-mediated leaf disc transformation used to introduce the Anti-LeERF2 gene into tomato (Lycopersicon esculentum cv. No.4 Zhongshu) was studied. The transformed plants obtained under the selection pressure of Kanamycin were identified by the PCR analysis, dot blot and Southern blot, which showed that the target gene was transferred into the plants and 25 antisense LeERF2 transgenic tomato plants were produced. In addition, the efficient tomato transformation system was developed. The result of Northern blot indicated the mRNA of LeERF2 in fruit exocarps of antisense plants was lower than thatof wild type.

Key words: tomato, antisense LeERF2 gene, tomato gentic transformation