FOOD SCIENCE ›› 2021, Vol. 42 ›› Issue (14): 159-164.doi: 10.7506/spkx1002-6630-20200605-073

• Component Analysis • Previous Articles     Next Articles

Authentication of Organic Tomatoes by Nitrogen Stable Isotope Ratio Mass Spectrometry and Liquid Chromatography-High Resolution Mass Spectrometry

WANG Shicheng, LI Guochen, WANG Ying, LI Bo, GAO Ningxuan, SUN Ci, SONG Yang, WANG Yanhong   

  1. (Liaoning Agricultural Products Research Center of Environment and Quality Safety Control Engineering Technology, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China)
  • Published:2021-07-27

Abstract: Traditionally, authentication of organic foods requires measuring a variety of parameters before making a comprehensive evaluation, which suffers from the tedious sample preparation and low discrimination accuracy. To address this problem, in this study, a method of identifying organic tomatoes based on stable isotope ratio mass spectrometry (IRMS) and high performance liquid chromatography-high resolution mass spectrometry (HPLC-HRMS) combined with partial least squares discriminant analysis (PLS-DA) was proposed. Specifically, 94 tomatoes were collected, of which 50 were grown organically, while the rest were grown in traditional ways. Each sample was homogenized and freeze-dried before chemical analysis. Nitrogen isotope ratio (δ15N) data and HPLC-HRMS data were acquired. Taken into consideration of all measured factors, a PLS-DA model for discriminating organic tomatoes was established, which was used to select and confirm 13 representative indicators including δ15N, organic acids, and flavonoids. The accuracy of the model was 93.4%, which could meet the requirement for the identification of organic tomatoes. This study provides an advanced approach for discriminating organic tomatoes quickly.

Key words: organic tomato; isotope ratio mass spectrometry; liquid chromatography-high resolution mass spectrometry; partial least squares-discriminant analysis

CLC Number: