Study on Pretreatment Method for Determination of Oxalate in Vegetables by Reverse Phase High Performance Liquid Chromatography

Abstract

Abstract: A method was established for the determination of oxalate in vegetables by Reversed Phase High Performance Liquid Chromatography with a reversed-phase AtlantisTM C18 column(250mm×4.6mm ID, 5μm)as immobile phase. The general interfering factor NO3 in samples was eliminated by using the powerful anion exchange resin - Resin HZ201. When detected with an UV detector at 220nm, the calibration curve showed a linear range of 0.5～300μ gml with a coefficient of variation of 1.4%(n=10)and a detection limit of 23 ngml(SN=3). It was found that the method was sensitive, with high percent recovery(from 100.2% to 101.9%), little disturbance, convenient and low cost.

Key words: reversed phase high performance liquid chromatography, strong base type anion- exchange resin, disturbance, vegetables, oxalate -

DING Jian-Hua, LUO Ming-Biao, WANG Xing-Xiang, TU Qi-Wei. Study on Pretreatment Method for Determination of Oxalate in Vegetables by Reverse Phase High Performance Liquid Chromatography[J]. FOOD SCIENCE, 2005, 26(12): 172-175.

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Study on Pretreatment Method for Determination of Oxalate in Vegetables by Reverse Phase High Performance Liquid Chromatography

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