At Room-temperature in 5 Minutes, A Rapid Method for the Determinaion of Protein in Corn

Abstract

Abstract: According to the characteristics that protein in corn is soluble in alcohol (90%～93%). The protein in corn was extracted with alcohol (90%) and coloured with basic solution of CuSO4 the absorbance was measured at 550nm. The optimum reaction of protein in the system was studied and a new rapid method, at room-temperature, for determining protein in corn was developed. The results showed two facts: ①The method needs no heating, centrifuging or suction and the test is simple and rapid, economical and safe in comparison with other rapid methods. ②The method has a high precision (RSD 0.38%), and fair reclamation (97.0%～101%). The results obtained by this method agree well with those obtained by the kjeldahl method.

Key words: , corn； protein； room-temperature； rapid determination；

LU Xiu-Heng, Lu-Jin-Chang, ZHU Mei-Meng. At Room-temperature in 5 Minutes, A Rapid Method for the Determinaion of Protein in Corn[J]. FOOD SCIENCE, 2006, 27(7): 218-221.

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At Room-temperature in 5 Minutes, A Rapid Method for the Determinaion of Protein in Corn

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