FOOD SCIENCE ›› 2004, Vol. 25 ›› Issue (1): 81-84.

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Extraction Isolation and Purification of Water Soluble Polysaccharides from Peach

 CHEN  Liu-Yong, MENG  Xian-Jun, KONG  Qiu-Lian, SONG  Li-Feng   

  1. 1.Food college of ShenYang Agriculture University;2.Centre of the Procession andPreservation of Agriculture Product of ShangHai Academy of Agriculture Science;3.Department of Technology of Biological Appliance of Liaoning Technological Institute of Economic Vocation
  • Online:2004-01-15 Published:2011-10-24

Abstract: The water soluble polysaccharides of peach were extracted in hot water, then isolated by ethanol. The proteinin polysaccharides was removed by Sevag way and the pigment in polysaccharides was removed by H2O2. The water-solublepolysaccharides of peach which color was pure white were got. HTP were further purified to HTP1 and HTP2 with DEAEcolumn chromatography. HTP1 and HTP2 were purified with Sephadex-300 column chromatography respectively, howeverwere singular peak. The gel filtration chromatography and paper chromatography proved that HTP1 and HTP2 were purematerial.

Key words: peach, polysaccharides, isolation, purification