Primary Experimental Research of Calorimetry on Pesticide Detection with Immobilized Enzyme

Abstract

Abstract: A calorimetric flow injection analysis system for detection of pesticide residue is described based on the theory ofenzyme reaction. This system consists of a peristaltic pump, a thermostat, Thermocouple temperature sensor, a high precisionmultimeter and a computer. The reaction mix solution was controlled at constant temperature when it flows through thermostat,then the enzyme reaction takes place in adiabatic cell. Constant temperature of the thermostat was realized using thermoelectrics.Constant temperature effect of the system is very good; the enzyme reaction heat can be detected by flow injection analysis andthe application of this system in pesticide detection can be validated.

Key words: wheat-esterase, calorimetry, flow injection analysis, pesticide detection, thermostat

ZHAO Xiao-Lei, ZHENG Yi-Hua, WANG Fang-Fang, HUA Ze-Zhao. Primary Experimental Research of Calorimetry on Pesticide Detection with Immobilized Enzyme[J]. FOOD SCIENCE, 2004, 25(2): 127-130.

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Primary Experimental Research of Calorimetry on Pesticide Detection with Immobilized Enzyme

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