Assay Study on Rapid Separation and Purification of Saponin Extract from Momordica charantia L.

Abstract

Abstract: The rapid routine assay of separation and purification on the extraction of saponin by alcohol from Momordicacharantia L. was studied in this paper. The solvent concentration, extraction period and the ratio of elution solution were consideredto ensure an optimized technological process to extract and isolate saponin from Momordica charantia L. Rg1(from Ginseng) wastaken as control.From the maxmium absorbance wavelength selected between the two UV spectra of saponin from Momordicacharantia L. and Rg1, a standard curve of Rg1 was made to determine the total content of saponin in the extracted products.

Key words: Momordica charantia L., saponin, separation and purification

CHEN Xun, YU Hai-Ning, TANG De-Song, HONG Jun, SHEN Sheng-Rong. Assay Study on Rapid Separation and Purification of Saponin Extract from Momordica charantia L.[J]. FOOD SCIENCE, 2004, 25(2): 114-117.

[1]	XIANG Ting, XIA Chen, LIU Jianhua, WANG Chaoran, SHEN Jianfu. Separation, Structural Identification and Anti-tumor Effects of New Compounds from Cordyceps militaris [J]. FOOD SCIENCE, 2021, 42(8): 235-242.
[2]	LI Yujing, SUN Liping, ZHUANG Yongliang. Purification and Thermal Stability of Geraniin from Rambutan (Nephelium lappaceum) Peels [J]. FOOD SCIENCE, 2021, 42(15): 44-49.
[3]	SHA Yuhuan, MAO Xiaoying, WU Qingzhi, ZHANG Jian, CHENG Weidong. Flavonoid Composition and Antioxidant Activity of Diaphragma juglandis Fructus [J]. FOOD SCIENCE, 2021, 42(12): 91-98.
[4]	CUI Xiaoying, PENG Xinyan, HE Hongjun, ZHANG Min, ZHANG Xiaotong. Separation, Purification and Enzymatic Characterization of Polyphenol Oxidase from Taro [J]. FOOD SCIENCE, 2021, 42(12): 107-115.
[5]	WANG Siqi, HU Yanbo, ZHAI Liyuan, SHI Zenghui, ZHAO Jun. Extraction, Purification and Structure Analysis of Soluble Acidic Polysaccharides from Soybean Dregs [J]. FOOD SCIENCE, 2021, 42(10): 52-57.
[6]	HUANG Qinqin, TIAN Yaping. α-Glucosidase and Dipeptidyl Peptidase IV Inhibitory Activity and Peptide Composition of Porphyra yezoensis Protein Hydrolysate [J]. FOOD SCIENCE, 2020, 41(24): 110-116.
[7]	ZHOU Yang, LI Lu, LÜ Ying. Effects of Roasting, Steaming and Extrusion on the Flavor and Bitterness of Quinoa [J]. FOOD SCIENCE, 2020, 41(20): 263-269.
[8]	LIU Zhijun, LIN Ling, LEI Zhengyan, ZENG Wenzhi, CHENG Fujian, LIN Quannü, YANG Jiangfan. Effect of Theasaponin on Blood Glucose in Streptozocin-Induced Diabetic Rats [J]. FOOD SCIENCE, 2020, 41(19): 179-184.
[9]	JIANG Jing, DU Renpeng, GUO Shangxu, ZHAO Dan. Separation, Purification and Biochemical Properties of Exopolysaccharides from Weissella confusa [J]. FOOD SCIENCE, 2020, 41(1): 9-15.
[10]	WANG Xiaohong, LI Yuanyuan, DAI Yufeng, WANG Jingfeng, LI Zhaojie, XUE Changhu. Mechanism of Improvement of Bone Mineral Density in Ovariectomy-Induced Osteoporotic Mice by Sea Cucumber Saponin [J]. FOOD SCIENCE, 2019, 40(5): 124-129.
[11]	LU Hongyan, YANG Hang, FANG Li, LIU Shimeng, MIN Weihong. Isolation, Purification and Structural Identification of Antioxidant Peptides Derived from Enzymatic Hydrolysis of Pine Nut Kernel (Pinus koraiensis Sieb. et Zucc.) Albumin [J]. FOOD SCIENCE, 2019, 40(24): 40-45.
[12]	HAN Yuzhu, LI Pinglan, HE Qin, ZHAO Zhanzhan, LIU Xuewen, YANG Yemei. Optimization of Extraction Conditions, Separation and Purification of Antifungal Peptides from Hot Pepper Seeds [J]. FOOD SCIENCE, 2019, 40(24): 258-264.
[13]	YU Shuqi, BAO Xiaoqing, LIANG Mingtsai, JIN Chenzhong, TIAN Wei. YU Shuqi, BAO Xiaoqing, LIANG Mingtsai, JIN Chenzhong, TIAN Wei [J]. FOOD SCIENCE, 2019, 40(20): 286-292.
[14]	CHEN Jiali, TAN Mengxia, ZOU Lisi, LIU Xunhong, CHEN Shuyu, SHI Jingjing, WANG Chengcheng, MEI Yuqi. Saponins in Panacis japonici Rhizoma as Analyzed by UFLC-Triple TOF MS/MS [J]. FOOD SCIENCE, 2019, 40(14): 249-258.
[15]	HE Pingping, WEI Jingliuyi, JIANG Zedong, ZHU Yanbing, NI Hui, LI Qingbiao. Composition and in Vitro Immunostimulatory Activity of Low-Molecular-Mass Fractions of Chemically Degraded Ascophyllan from Ascophyllum nodosum [J]. FOOD SCIENCE, 2019, 40(11): 139-145.

Assay Study on Rapid Separation and Purification of Saponin Extract from Momordica charantia L.

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments