Study on Protein Obtained from Denatured Soyben Meal with Proteinase

Abstract

Abstract: The denatured soybean meal was obtained from the high temperature desolventized bean. At the same time thedenatured soybean meal hydrolyzed with proteinase was studied systematically by using orthogonal experiment method.Fer thedenatured soybean meal hydrolyzed with basel proteinase,the effets of temperature,pH value,time,substrate corcentraction andenzyme/substractrate during the hydrolysis were studied. The optimum conditions of the hydrolytic high temperature denaturedsoybean meal was obtained. The results provided the reference values for the study of hydrolysis of the other proteinmaterials.Results indicated that the optimum extrodting conditions of the denatured soybean meal by basic proteinase were asfollowings:temperature:50℃,time 6h,substract conurtraction 11.0%,enzyme/substract 10000U/g.In such conditions 69.34%protein in denatured soybean meal would be hydrolyzed and digested.

Key words: the denatured soybean meal, hydrolysis, protein

LIU Fang, WANG Sui. Study on Protein Obtained from Denatured Soyben Meal with Proteinase[J]. FOOD SCIENCE, 2004, 25(3): 89-92.

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Study on Protein Obtained from Denatured Soyben Meal with Proteinase

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