The effects of ultrasonic fields on the activities and secondary structures of Kunitz trypsin inhibitor (KSTI) andBowman-Birk trypsin inhibitor (BBTI) from soybean were studied by measuring the changes of inhibitory activities, sulfhydrylbonds and far-UV circular dichroism (CD) spectra. It was found that the far-UV CD spectra of both SKTI and BBTI show asingle negative peak at around 200nm. The negative minimum [θ]200nm of SKTI was measured as -2545 deg•cm2•d/mol andthe secondary structure was composed of β-sheet (22.5%), β-turn (16.2%) and random (61.4%). The negative minimum [θ]200nm of BBTI was measured as -797 deg•cm2•d/mol and the secondary structure was composed only of β-sheet (52.6%)and random (47.4%). By the treatment at 65% amplitude of ultrasonic field and 11min period, [θ]200nm of KSTI was changedas-1827 deg•cm2•d/mol. When the treatment period was extended to 20 min, The β- turn and random coil content of KSTIwere decreased to 10.8% and 54% respectively and β-sheet was increased to 35.2%. About 71.5% of disulfide bonds werefound transformed to sulfhydryl bonds and 55% of inhibitory activity inactivated. Whereas BBTI show more stable than KSTIunder ultrasonic fields in its inhibitory activity and secondary structure. With 20min period treatment at 65% amplitude ofultrasonic field.The [θ]200nm of BBTI was only changed as -700 deg•cm2•d/mol. The β-sheet, random structures and theinhibitory activity of BBTI were kept unchanged basically and only about 5.29% of disulfide bonds were transformed. Hence itis suggested that ultrasonic fields lead the reduction and breakdown of disulfide bonds of SKTI, and then lead the alteration of thesecondary structures of KSTI, and finally, inactivate the inhibitory activity of KSTI.

Two soluble extractions of polysaccharides named Mb and Mc were extracted from Candida tropicalis andSchizosacchromyces octosporus by chemical precipitation method. Thin layer chromatagrathy showed that Mb was mainlycomposed of mannase and Mc was composed of mannase and galactose. Protein analysis revealed that Mb and Mc consisted ofpolysacchrides and proteins and amino acid analysis proved that Ma1 contained 7 kinds of essential and 9 kinds of non-essentialamino-acids. Pyrogallol autooxidation analysis revealed that Mb and Mc showed anti-oxidative properties in vitro.

Recently, scientists have found that milk and some other proteins were possible precursors of many different biologicalactive peptides. These peptides are inactive within the sequence of the precursor proteins and can be released from theprecursor when treated with proteinase under proper conditions such as pH, temperature and ionic concentration. Up to now,several kinds of biological peptides have been found from the proteolysis solution of nutritious or seed proteins. Because ofthese proteins were very cheap, rich in resources and safe for health, the bioactive peptides thereby have had commercialpotential, also been suitable to scale up. Therefore the degree of hydrolysis, digest conditions and the relationship between themacromolecule proteins substrates and the proteinases were investigated in several experiments in this paper. The resultsshowed that when the concentration of the substrate increased over some point, the rates of reactions will decrease on thecontrary. It was obviously deferent to the Michaelis-Menton Equation. It showed that the interactions between enzymes andsubstrates of macromolecular proteins might be more complex.

In this paper, the crude polysaccharides from the fruitbody of mushroom Dictyophora rubrovolota were obtainedby extracting with hot water and precipitating with alcohol. The free proteins were removed from crude polysaccharides, andthen the polysaccharides were isolated and purified by Sephadex G-15 gel column chromatography. Three pure polysaccharides(Dr-1, Dr-2 and Dr-3) were obtained by precipitating with different concentration alcohol. By using the TLC method, we provedthat the three fractions were all composed of galactose (Gal), glucose (Glu), mannose (Man) and xylose (Xyl).

Taking catechol as substrate,this article made a research on the characteristics of polyphenol oxidase(PPO)ofbanana by spectrophotometer ,including its catalytic characteristics, the optimum temperature, the optimun pH, the thermody-namic stability of enzyme and so on. The results showed that the optimum temperature and pH were 25℃ and 5.0 respecfively.ThePPO was inactivated at 75℃ for 5min and the extinct condition of PPO was 20s at 100℃.The PPO had isoenzyme in its mixture.

The effects of the high pressure solution treatment on the soy protein isolate (SPI) edible films properties had beenstudied. The results showned that the treatment could improve the stability of soy protein isolate solution, make the tensilestrength of films increase and the elongation, oxygen permeability decrease.The instant ratio in hot water was kept stability andthe film surface fine and smooth.

Copper/zinc superoxide dismutase (Cu,Zn-SOD) from black-bone chicken erythrocytes has been purified bychloroform-ethanol fractionation through acetone precipitation and DE-32 chromatography. The results showed that the spe-cific activity of the enzyme was 11,954U/mg , and its purification factor was 334.71. This enzyme showed two identicalsubunits.The molecular weight was about 32,000,and the identical subunit molecular weigh about 16,000.The ultraviolet ab-sorption peak of the enzyme was found at 260nm .The enzyme was sensitive to KCN and H2O2.The enzyme optimum pH wasfrom 6 to 9 and stable below 60℃.

Static adsorption test and static de-adsorption test were carried out to screen the best macroporous resin for the Cassianomame polphenols.The single factor tests, orthogonal experimental designs and variance analyses were applied to optimize themanipulation parameters of macroporous resin. The results indicated that macroporous resin S-8 possessed the strongest ad-sorption ability to Cassia nomame polyphenols among the various resins studied, in addition to an easy de-adsorption property.The optimum adsorption conditions were A3B2C3, i.e. feeding rate 2.5BV/h, concentration of feed 4mg/ml and pH value 6.5. Theoptimum elution conditions were A2B3C2D1, namely elute volume 3.5BV, elute concentration 75%, elution rate 2BV/h and pHvalue 8.

The antioxidant activity of Rhodotorular glutinis pigment was determined by the capability of eliminating hydroxyradical and preventing linoleic acid lipid peroxidation in the text. VE as a standard antioxidant was used to contrast with sample.The results showed that Rhodotorular glutinis pigment and VE had similarly eliminate effect on hydroxy radical in a higherconcentration, but in a lower concentration , the Rhodotorular glutinis pigment had a better activity .And in this experiment,Rhodotorular glutinis pigment only in high concentration could inhibit linoleic acid lipid peroxidation.

Antioxidative activities of different solvent extracts of Ear of Staghorn Sumae were studied by 1,1-diphenyl-2-picryhydrazyl (DPPH) free radical method, and the antioxidative activities of ethanol extracts with other materials were studiedby Na2S2O3-I2 litrimetric method using lard as substrates. The results show that the antioxidative activities of different solventextracts decreased in the order: ethanol solution >ethyl acetate > n-hexane. Antioxidative activities were strengened whenincreasing the usage of extracts. Ascorbic acid and Citric acid had synergistic antioxidative activities with ethanol extracts, butamino acid had no synergistic effect. The antioxidative components of Staghorn Sumae is Ear are suggested to be polyphenolicsubstances.

Mechanism of the enzymolysis of muscle proteins from Harengula Zunasi Bleeker by compound enzyme that obtainedfrom mixture with papain and flavorease was studied. The optimum conditions of compound enzyme for enzymolysis muscle proteinare at enzyme consistency 7.5×103IU/g protein, temperature 45℃, pH6.5 and substrate consistency 5.5g protein % throughorthogonal trial with the constant time of 2 h. Under the optimum conditions, there are 3 linear relationship models expressed asDH%=0.026×[hydrolysis time]+8.85 (r=0.98), TFAA (total free amino acids of hydrolysate) =0.16×[hydrolysis time]+38.76(r=0.99) and MLPL(mean length of peptide linkage of hydrolysate)=-0.019×[hydrolysis time]+10.79(r=-0.96).The analysisof free amino acids revealed that compound enzyme has the strongest hydrolysis specificity toward the peptide linkages suppliedby cysteine and phenylalanine+tyrosine and the lowest hydrolysis specificity toward the peptide bonds supplied by tryptophane,proline, asparaginic acid, serine, glycine and glutamic acid. Meanwhile, the amino acids arriving at maximum hydrolyzing capac-ity before 210 min may have participated in the forming of the new peptide bonds at certain extent after arriving at themselvesmaximum hydrolyzing capacity. The determining of nitrogen content revealed that there is significant difference of the moleculesize of nitrogenous compositions among centrifugal hydrolysates, 0.45 μm hydrolysates and 0.22 μm hydrolysates at sametime of a range from 30 to 210 min because their nitrogen content has significant difference. The determining of molecularweight revealed that there is no significant difference in mean of relative molecular weight of all centrifugal hydrolasates and therelative molecular weight being from 300 to 1200 is covered 66% to 78% of total of the molecule number in centrifugal hydroly-sates. It is possible to give fundament and recommendations for the all-around utilization of Harengula Zunasi due to this work.

To get high content of docosahexaenoic acid (DHA,ω-3), the effects on the Cephalosporium sp. mycelium’s fattyacid composition of strain storing and culturing conditions were studied. The strgins containing microorangism were stored at 20℃、4℃and-20℃ for different times respectively. The fatty acids were analyzed by GC. The results showed that the fatty acidcomposition was affected by the storing temperature、storing time no matter how the strain was activated on new agar beforefermentation. If the strain was cultured on new agar before fermentation, the 10d period was the best storing time to get highsDHA content. If not cultured, to the 20℃, the best time was 20d; to the 4℃, the best time was 25d and to -20℃, it was 5d. Thepolyunsaturated fatty acid (PUFA) changed the same tendency as the DHA’s but at 4℃ not so. At 20℃, the index ofunsaturated fatty acid (IUFA) and the 18:3 (α-)/18:2 changed with the same tendency as the DHA’s. Storing the strain at -20℃, the PUFA、IUFA and DHA fluctuated at the lowest range. This study could give a theoretical reference for producing highyield of DHA by microorganisms.

A method for urea adsorption by chitosan-Zn(Ⅱ) complex was studied.The optimal conditions were obtained fromexperiments.The adsorption capacity of the complex for urea was 83.97mg/g under room temperatur and pH value between 6～7,while the original concentration of urea was 2.0mg/ml.The adsorption time was 3.0h.

The isolation,definition and characteristics of lactic bacteria in the nature fermentation of pickling mustard werestudied.After the identification of shape and culture and biochemistry tests,it was determined that six isolated kinds of lactic acidbacteria were differentiated as Leuconosioc mesenteroids,Lectobacillus fermentum,Lectobacillus plentarum,Pediacoccuscererisias,Yeast and Lectobacillus hrevis.According to their characteristics,the major bacteria of all the natural fermentationprocess were determined for better quality and optimum conditions.

L.plantarum 6003 was used as LABstarter added in the broth with E.coli O139 and S.typhimurium being representedenterobacteria to imitate dry fermented sausage.The effects of garlic with different concentrations added to the imitating broth werestudied.In revealed that 0.05% and 0.1% garlic could effectively delay the growth of E.coli O139 and S.typhimurium espectively while1% garlic could inhibit completely the growth of the enterobacteria,but 1% garlic had no inhibition on the LAB starter.

The denatured soybean meal was obtained from the high temperature desolventized bean. At the same time thedenatured soybean meal hydrolyzed with proteinase was studied systematically by using orthogonal experiment method.Fer thedenatured soybean meal hydrolyzed with basel proteinase,the effets of temperature,pH value,time,substrate corcentraction andenzyme/substractrate during the hydrolysis were studied. The optimum conditions of the hydrolytic high temperature denaturedsoybean meal was obtained. The results provided the reference values for the study of hydrolysis of the other proteinmaterials.Results indicated that the optimum extrodting conditions of the denatured soybean meal by basic proteinase were asfollowings:temperature:50℃,time 6h,substract conurtraction 11.0%,enzyme/substract 10000U/g.In such conditions 69.34%protein in denatured soybean meal would be hydrolyzed and digested.

Studies on the technics of extracting soybean protein from de-oiling soybean meal using reverse micellar system bis-(2-ethylhexyl)sulfosuccinate (AOT)/isooctane. The effect of pH, W0, AOT concentration, KCl concentration, extraction time,extraction temperature was investigated.

In Zao pepper, a naturally fermented lactic pepper, lactic-acid bacteria(LAB) is a dominant microflora, and a few ofyeasts also play a important role. It is suitable for the production materials of Zao pepper to choose the mature red pepper inearlier stage with the thick and solid tissue or the hard and fragile texture. In the production of Zao pepper through the newprocessing technology of the direct bottle-filling fermentation, either the selective inhibitation through the acid regulation or theantisepsis, or the intensification of dominant microflora through the artificial inoculation after reducing microbe on the pepper issurface, may completely guarantee both the safe fermentation and the quality improvement of the product.

We found that the pressure, temperature and pressure holding time are believed to be the major factors for sterilizationof high hydrostatic pressure through the studies of the effects of external factors on Bacillus subtilis of high hydrostatic pressureinactivation. Response surface methodology (RSM) was employed to optimize the process variables of there major factors.Result showed that the condition to sterilize Bacillus subtilis Of six log cycles such as temperature (31.10～59.03℃), pressure(435.23～562.21 MPa ), pressure holding time (10.11～19.53min ), and process parameter of ten groups were optimized, whichwas also verified experimentally.

Oligosaccharide was extracted from Stachys geobombycis with hot water and alcohol. The Stacgts geobombycis wasboiled at 80℃ for 2.5 h with liquid-to-solid ratio of 10:1 ml/g and the content of total polysaccharide in supernatant fluid could reach80% after filtration. Alcohol in different concentration was used to treat the filtrate liquor through a fractionated precipitationway to get the different oligosaccharide, which was then used to treat wheat to induce the resistance to wheat stripe rust.Results show that the alcohol in higher concentration could get the oligosaccharide, which is more helpful to induce the resis-tance of wheat to stripe rust.

The effects of temperature and pressure on the permeate flux of yellow rice wine were studied by tubular UF unit, thechanges of its quality indexes were investigated. The average permeate flux of yellow rice wine is up to 75 kg/m2•h at 0.30MPa and 40℃ with organic membranes of 50000 dalton pore size. The removing rate of total conolies is over 98%, insolublesolids is all removed, retention of crude protein is 21.55%. Ethanol content is almost constant, retention of free amino acids isdifferent, but Retention of total amino acids is up to 86.76%. Old membranes can be cleaned by soaking for a longer time.

This production was made of the two special local products; Tibet Tricholoma and Tibet highland barley,hemi-solidferment was used。The conditions of sugar engendering and fermentation are the emphases in this research:the time of sugarengendering is 24h、the quantity of microzyme is 0.8%、highland barley /Tricholoma is 50/1、fermentation time is 40d、themode of fermentation temperature is 28-29℃～32-24℃、the quantity of water is 125%。

In this paper sucrose polyesters was synthesis by two step method from cotton seed oil and sucrose. The optimumcondition to form Sucrose Polyesters was discussed.The measurements of such as IR,DS,Iodine value,acid value ,soaponficationvalue etc.

With the chitosan as raw material, we make a agent that can deal nature vinegar. The agent can remove the muddything and the precipitation of nature vinegar, and produce a good nature vinegar that is amber and transparent. The agent doesnot have the poisonous side-effect, and does not pollute the environment.

The effects on the brewing technology of Korla pear brandy with special flavor and high quality by using different stateof Korla pear as raw material, through mashing、ingredient adjusting and fermenting at different tempture by using dry yeastswere studied. Compared with the dry wine yeast, a 10L-scale fermentation in Kuerle pear brandy was performed by usingscreened yeast according to the brewing technology.

Passiflora edulis is the farmer’s tropical fruit with unique fragrant used in fruit juice processing.The paper hasresearched a species of compound drink that was made from Passiflora and Wenzhou Orange,increase Orange juice and Daucuscaroto juice to adjust color and taste.Adopting the Orthogonal test is to find the best formulation and the stalbilizer of the beverageaccording to a synthetical mark of tint,taste,fragrance and sediment.

Supercritical CO2 extracted euchroma(Royle) Johnst was investigated and compared with organic solvent extraction.The resulted showed that the yield is up to 4.2% at 32℃ 27MPa by SFE and the yield of Naphthaquinonic compounds is up to3.59% at the same conditions. More components is extracted at SFE than that at organic solvent extraction, and red pigmentcontains less non-Naphthaquinonic compounds at SFE than that at organic solvents extraction.

Based on less content of active ingredients of Chinese herb, crystal ingredients jamming pipeline, and so on, we took extractof ginkgo biloba L. as experimental material to investigate the effect of extracting and crystallizing pressure, temperature and timeon the purity of ginkgolides extracted and crystallized by supercritical CO2 via single factor experiments. The results showed thatextracting and crystallizing pressure 20MPa, temperature 55℃, time 90min, the purity of ginkgolides was more than 85%.

The fumonisin mycotoxins, originally isolated in 1988, are produced by several species of the genus Fusarium, whichhave considerable attention on fond safety due to their occurring worldwide and high toxin. In a world-based survey of fumonisinlevers in maize and maize-based products, over 90% of labs that reported results used precolumn derivatisation and HPLC fordetermination. In this paper the research progresses on determination of fumonisin in food by precolumn derivation and HPLCare reviewed. The methods of extraction, purification and derivation of food samples, the selection of mobile phase and theprecision are also discussed.

Several methods were selected to determine the content of flavonoids in corn silk, while satisfying wave length wasdecided by means of UV-visible photometer curves of flavonoids and its monomer in corn silk, as well as rutin at the range of200600nm after reacting with different regents , and comparison of experimental results were carried out. The results showedthat Al（NO3）3 colorimetry is not suitable to determine content of flavonoids in corn silk. While AlCl3 colorimetry is a simple,accuratemethod which can be used to determine content of corn silk flaovonoids with rutin as reference substance.

The water distillation-extractor was used for extracting the volatile substances of white P. nigrum L.in Hainan. Theaverage oil yield obtained was 4.7 % . 35 components were identified by GC / MS method. The contents of the identifiedterpinene compounds make up 88.70% of the total volatile substances detected.

A rapid SFC method for determination of orotic acid in cow milk is described. The mobile phase was a mixture of CO2and methanol (contained 0.25% TFAA) at a rate of 2.0ml/min,and a packed column was used. The UV detector was at awavelength of 280nm.The calibration curve was linear in the range of determination(r=0.9991). The RSD of peak area andretention time were 2.33% and 0.47% respectively. The recovery was 101.9%±0.92%. The analysis was completed within 3minutes. The method was simple and it was easy for handling sample. It could be used in rapid analysis.

An improved method for determination of 3-chloro-1,2- propanediol and 1,3-dichloro-2- propanol in expanded foodproducts was presented. Chloropropanols were extracted with diethyl ether in reflux extractor, then purified with methanol incentrifuge tube. The identity of N,O-bis(trimethylsily)trifluoroacetamide derivatives was confirmed by capillary gas chromatog-raphy with flame ionization detection. The detection limit of the method was 0.01μg/g . Recoveries of chloropropanols rangedfrom 85 to 95% .

An analysis was performed by high performance liquid chromatography using NH2-Lolumn, and acetonitrile solvent(acetonitrile: water=70∶30). Refractive Detector, the quantity of the constituents was determined by external standard method. High-maltose syrups was formed from potato starch with enzyme, and a new analytical method of high-maltose syrups was put forward.

Separation of cis,trans-piceid and cis,trans-resveratrol by High-performance Liquid Chromatography was studied.When mobile is ACN and water were used with gradient, cis,trans-piceid and cis,trans-resveratrol could be separated by HPLCeffectively in 25min.

By means of the existence of Non-ion surfactant to enhance its micellar and sensitivity.This article mainly discussedtheimprovement of sensitivity of color reaction by the method of spectrophotometry. And it also summarized the research and the newmethod of determination of miniscule amount of iron in monosodium glutamate by spctrophotometry method by using the highsensitive and selective surfactant-Chrome azurol S CTAB .This determination of linear range was 014μg/25ml. And its limit ofproof-test was 8.4×10-8 g/ml. This method also has a good fidelity and high precision with easy operation and satisfied test results.

The crude phycobiliproteins were got from water extraction of smashed cells of Gracilaria lemaneiformis wereprecipitated in ammonium sulfate. Phycoerythrin could be separated through chromatographed the crude phycobiliproteins bySephadex G-100 column, and it appeared only one band in the polyacrlamide gel electrophoresis. Molecular weights of subunitsof the phycoerythrin are 18000Da and 24000Da that were measured by SDS-PAGE. The PE possessed an absorption maxi-mum at 570nm and it belongs to R-PE type Ⅰ.The scavenging activities of the crude phycobiliproteins and the PE on OH ofFenton reaction and O2• f Luminol reaction were studied.The results showed that the crude phycobiliproteins and the PE bothcould scavenge free radicals, but their abilities were different.

The study was about whether and how much flaxseed oil reduces the blood lipids of SD rats. Total cholesterol (TC),Triacylglycerol(TG), High density lipoprotein cholesterol(HDL-c) were measured in the 28th day for all groups. The results showedthat flaxseed oil can not only reduce TC and TG value in hyperlipid rats significantly, But also improved HDL-c significantly.

Objective To study the protective effects of flavonoids of ginkgo biloba on chronic alcohol induced oxidative damageon rat. Methods The rat was treated by gastric incubation with alcohol. After administered with preventive medicine flavonoidsof ginkgo biloba, the activity of ALT(Glutamic-Pyruvic Transaminase, ALT)、AST(Glutamic Oxaloacetic Transaminase, AST)were measured in rat serum, meanwhile, the concentration of ROS(Reactive Oxygen Species)、 Glutathione (GSH)、Malondialdehyde (MDA) on the 30th day and 60th day of the experiment, respectively were determined to indicate serumoxidative-damage. Result The preventive experiment display that on the 30th day, the low and high doses flavonoid of ginkgobiloba can significantly decrease the high ROS、ALT、AST and MDA levels induced by alcohol (p<0.01);moreover, the highdose can increase GSH level reduced by alcohol markedly(p<0.01) on the 60th day. Conclusion flavonoids of ginkgo biloba havesome protective effects on chronic alcohol induced oxidative damage on rat serum.

Objective: To discuss the inhibiting mechanism of LSPC on the biosynthesis. Methods: Tyrosinase inhibiting rate isdetermined and Lineweaver-Burk curve is made by determining the tyrosinase activity before and after drug(LSPC) interfer-ence oxidaut of method, on which we deduce the inhibiting types of LSPC.Results: LSPC can inhibit the tyrosinase activityremarkably with the half inhibiting concentration IC50 1.155g/L, and can effectively inhibit the transition from dopa, the medial-production when melanin is formed , to melanin with the half inhibiting concentration IC50 1.750g/L.Conclusion: LSPC can inhibitthe tyrosinase activity and it is the competitive inhibitor of tyrosinase.

The antioxidant activity of shaddock peels extract(SPE) in rape oil system and its immunomodulatory effects on normalmice was studied. Grapefruit Skin extract showed significant antioxidant activity in rape oil system at concentration of 500×10-6,and VC had synergistic antioxidant effect to SPE in rape oil system. but when the concentration increased to 1000×10-6 and 2000×10-6, it showed pro-oxidant effect, and SPE can also activate serum SOD and reduce the content of MDA of mice significantly,and the concentration-effect relationship was obvious; It was found that Grapefruit Skin extract (Po,100,200mg/kg•d×7d)can significantly intensify DTH caused by DNFB, and SPE (100mg/kg•d,200mg/kg•d, ×15d) can improve phagocytosingfunction of peritoneal macrophage(PMφ) by 22.94%, 53.96% respectively;

Kuerle”pear treated with CMC edible coating carrying yeast was used as raw material in this experiment. Throughthe study on effects and physiology of storage, a kind of natural, no-toxic, highly efficient biological coating was found out. The resultsshowed that all the treatments had restrained respiratory intensity, chlorophyll decomposition, PPO activity and MDA accumulation,reduced rot rate and postponed ripeness and softness. CMC+Yc-b combined with yeast could strengthen antiseptic effect remark-ably. Stored at 15℃,rate of rotting and loss of “kuerle”pear still remained low at 5% after two months storage.

The culture conditions for the production of antagonist by Bacillus sp. X-98-2 and the effect on preservation of mangowere investigated. The results showed that the optimal culture conditions were 0.3%glucose, 0.5% peptone ,0.3%immersed beefcream, 0.1% NaCl and 0.1% (NH4)2SO4, pH7.0, at 28℃ for 48h. The leavening had a good effect on preservation of mango inmany aspects, such as preventing fruit stem-end falling off, postponing the seedcase to turn yellow, inhibiting the metabolism,reducing the respiratory intensity, keeping the weight, and so on.

In order to preserve green peppers, tomatoes, sausages and salted meat fresh, zein films were used and differentplasticizers were added. The results showed that the best making-film solution were 1g zein, 10ml 80% ethanol, 0.2ml oleic acidand 0.3g shell-lac for sausages and salted meat; 5% glycerine 0.8ml for green peppers and 0.5ml for tomatoes. The zein filmscould prolong the foods’shelf life effectively.

Mammal milk is not only enriched in nutriments, but also contains abundant biological active substances. There are alsolive cells in milk, too. These biological active substances play important roles in metabolism, growth, development, immunization,antimicrobes, etc either of neonates or of young animals. Some biological active substances are closely related with develop-ment, differentiation and protection of mammary gland. The present paper devoted to the introduction of biological active sub-stance in milk, such as hormones, growth factors, enzymes, immunoglobulins, lactoferrin, milk fat globule membrane proteins,bioactive peptides and so on.

Lactoferrin, an iron-binding glycoprotein, has many biological functions. The latest hot-spot researches of lactoferrin havebeen reviewed on the activated lactoferrin to produce high levels of human lactoferrin (hLF) in the transgenic organisms andisolation and purification on a large scale. The prospects of the development and application of lactoferrin were also summed up .

Biotechnology-derived (BD) foods are those foods produced from gene modified organisms,which are developedby those processes whereby genes that are not endogenous to the organism are transferred to microorganisms,plants,oranimals employed in food production,or where the expression of existing genes is permanently modified,using the techniquesof eexisting genes is permanently modified,using the techniques of genetic engineering.It was briefly introduced the conceptof substantial equivalence for the assessment of safety for BD foods.Two key issues for the safety of BD foods,i.e.theinherent toxicity of the transgenes and their products,and unintended effects resulting from the insertion of the new geneticmaterial into host genome,were analyzed and discussed from the perspectives of both theoretic conjecture and reviews oftrial results.The status and challenges of current safety assessment methodologies for Bd foods were also smmarized.

Elaborate the situation and development tendency of China milk industry.The contest of China milk incustry willbe obey the discipline and keep the law.The development of China milk industry will be rely on visorosly develop of scienceand technology.Be aimed at the approximately idea roasting that the part of mass media have something to do with suckingtrade manufacture and the consumption respect,clarifying of the original adjust the mamma destroying harmful microor-ganisms ,exterminate bacterium mamma,acid breasts,lactic acid fungus beverage ,reduction breasts,premature deliverybreasts,the dairy products such as milk powder cheese and so on ,moreover it is correct “The illness is not standed bythe milk sugar ”doing the synthesis stateed .

The walnut and peanut milk using walnut, peanut and fresh milk as raw materials, The technology of fermentedwalnut and peanut milk was studied. The number of additives and culture sadded , the fermented temperature and time and theacidity controlled were sifted from the experiments. Then the optimum procedure and prescription to produce the product wereintroduced. It also compared its flavor and nutrition quality with the non-fermented product. The results showed that thefermented walnut and peanut milk was a nutritious and delicious fermented milk product.

The incubation of spongy vehicle carrier made of konjak after specific physical and chemical treatment with calciumrich, plearotus eryngi mycelia were mixed soya milk and edible fungi of high nutrient and low cost. The experiment provided amodel to produce calcium rich soymilk yogurt for the enterprises.

This paper expounded the technological problems and quality requirement of the fast frozen dumplings.Thecomparatively optimal process parameters have been established on the basis of single factor and orthogonal experiments inadditive ingredients,flour pH value and freezing speed to improve the quality of the said food.

Pugionium, a plant of cruciferae family,was both edible as food and usable as ingredient of the traditional Chinesemedicine. The analysis of its nutritious element showed that pugionium had richer nutrients compared with other vegetables, suchas protein, vitimin C and cellulose. As a result, it was of good nutrition and medical values. Some exploration on the processingprocedures of the series products was attached.