Studies on Separation and Scavenging Activities on Free Radicals of Phycobiliproteins from Gracilaria Lemaneiformis

Abstract

Abstract: The crude phycobiliproteins were got from water extraction of smashed cells of Gracilaria lemaneiformis wereprecipitated in ammonium sulfate. Phycoerythrin could be separated through chromatographed the crude phycobiliproteins bySephadex G-100 column, and it appeared only one band in the polyacrlamide gel electrophoresis. Molecular weights of subunitsof the phycoerythrin are 18000Da and 24000Da that were measured by SDS-PAGE. The PE possessed an absorption maxi-mum at 570nm and it belongs to R-PE type Ⅰ.The scavenging activities of the crude phycobiliproteins and the PE on OH ofFenton reaction and O2• f Luminol reaction were studied.The results showed that the crude phycobiliproteins and the PE bothcould scavenge free radicals, but their abilities were different.

Key words: gracilaria lemaneiformis, phycobiliproteins, separation, absorption spectrum, free radical

CHEN Mei-Zhen, ZHANG Yong-Yu, YU Jie, XIE Xiong-Bin. Studies on Separation and Scavenging Activities on Free Radicals of Phycobiliproteins from Gracilaria Lemaneiformis[J]. FOOD SCIENCE, 2004, 25(3): 159-162.

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Studies on Separation and Scavenging Activities on Free Radicals of Phycobiliproteins from Gracilaria Lemaneiformis

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