Study on the Polysaccharides Extraction from Mushroom Dictyophora rubrovalvata by TLC

Abstract

Abstract: In this paper, the crude polysaccharides from the fruitbody of mushroom Dictyophora rubrovolota were obtainedby extracting with hot water and precipitating with alcohol. The free proteins were removed from crude polysaccharides, andthen the polysaccharides were isolated and purified by Sephadex G-15 gel column chromatography. Three pure polysaccharides(Dr-1, Dr-2 and Dr-3) were obtained by precipitating with different concentration alcohol. By using the TLC method, we provedthat the three fractions were all composed of galactose (Gal), glucose (Glu), mannose (Man) and xylose (Xyl).

Key words: Dictyophora rubrovolota, polysaccharides, isolation, purification, TLC (Thin-layer-chromafography)

LIAN Bin, YU Jian-Ping. Study on the Polysaccharides Extraction from Mushroom Dictyophora rubrovalvata by TLC[J]. FOOD SCIENCE, 2004, 25(3): 43-45.

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Study on the Polysaccharides Extraction from Mushroom Dictyophora rubrovalvata by TLC

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