FOOD SCIENCE ›› 2004, Vol. 25 ›› Issue (3): 51-54.

Previous Articles     Next Articles

Studies on Purification and Properties of Superoxide Dismutase Isolated from Black-bone Chicken Erythrocytes

 ZHANG  Lan-Jie, XIN  Guang, ZHANG  Wei-Hua, YUAN  Qin-Sheng   

  1. 1.Department of Chemistry,Anshan Normal University;2.College of Life Science,East China University of Technology
  • Online:2004-03-15 Published:2011-10-24

Abstract: Copper/zinc superoxide dismutase (Cu,Zn-SOD) from black-bone chicken erythrocytes has been purified bychloroform-ethanol fractionation through acetone precipitation and DE-32 chromatography. The results showed that the spe-cific activity of the enzyme was 11,954U/mg , and its purification factor was 334.71. This enzyme showed two identicalsubunits.The molecular weight was about 32,000,and the identical subunit molecular weigh about 16,000.The ultraviolet ab-sorption peak of the enzyme was found at 260nm .The enzyme was sensitive to KCN and H2O2.The enzyme optimum pH wasfrom 6 to 9 and stable below 60℃.

Key words: Black-bone chicken, erythrocytes, cupper/zinc superoxide dismutase (Cu,Zn-SOD), purification, property