Determination of Orotic Acid in Cow Milk by Supercritical Fluid Chromatography

Abstract

Abstract: A rapid SFC method for determination of orotic acid in cow milk is described. The mobile phase was a mixture of CO2and methanol (contained 0.25% TFAA) at a rate of 2.0ml/min,and a packed column was used. The UV detector was at awavelength of 280nm.The calibration curve was linear in the range of determination(r=0.9991). The RSD of peak area andretention time were 2.33% and 0.47% respectively. The recovery was 101.9%±0.92%. The analysis was completed within 3minutes. The method was simple and it was easy for handling sample. It could be used in rapid analysis.

Key words: cow milk, orotic acid, SFC, determination

GUO Ya-Dong, MA Yin-Hai, LI Fan, ZHANG Yan. Determination of Orotic Acid in Cow Milk by Supercritical Fluid Chromatography[J]. FOOD SCIENCE, 2004, 25(3): 145-147.

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Determination of Orotic Acid in Cow Milk by Supercritical Fluid Chromatography

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