FOOD SCIENCE ›› 2004, Vol. 25 ›› Issue (3): 33-36.

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Purification, Identification and Anti-oxidative Analysis of Two Yeast Polysaccharides

 WANG  Yi-Hua, XU  Mei-Zhen, JIANG  Ping, HE  Zhao-Fan, XIONG  Lu-Yun   

  1. 1.Department of Biological Science and Technology,Tsinghua University;2.Department of Food Science,Jiangxi Agriculture University;3. College of Biology Technology,Guizhou University
  • Online:2004-03-15 Published:2011-10-24

Abstract: Two soluble extractions of polysaccharides named Mb and Mc were extracted from Candida tropicalis andSchizosacchromyces octosporus by chemical precipitation method. Thin layer chromatagrathy showed that Mb was mainlycomposed of mannase and Mc was composed of mannase and galactose. Protein analysis revealed that Mb and Mc consisted ofpolysacchrides and proteins and amino acid analysis proved that Ma1 contained 7 kinds of essential and 9 kinds of non-essentialamino-acids. Pyrogallol autooxidation analysis revealed that Mb and Mc showed anti-oxidative properties in vitro.

Key words: yeast, polysaccharide, thin layer chromatography, anti-oxidation