Comparison in Determination Methods of Vitamin B1,B2

Abstract

Abstract: Objective: To compare the determination methods of VB1, VB2. Methods: the three different methods of UV fordetermining VB1, VB2 are adopted. Results: According to the value of E, the contents were 98.3±2.51 for VB1 and 94.8±0.308 for VB2, the VB1 content of compound vitamin B tablets was 102.1±1.70. The average recovery for VB1 and VB2 were100.4 and 94.9 respectively. According calibration curve method: the concentration of VB1 and VB2 were 3～18 and 1～8μg/ml, showed good linear relationship. The results were 102.9±0.661 for VB1 and 98.4±0.152 for VB2. The dual wavelengthspectrophotometery: the content of VB1 was 04.8±1.97. Conclutions: The three methods could be used to the determination of VB1,VB2.They are simple, rapid, accurate, the precision are very good.

Key words: UV, vitamin B1, vitamin B2, compound vitamin B tablets

GUO Ya-Dong, MA Yin-Hai, LI Yan-Hua, LI Fan. Comparison in Determination Methods of Vitamin B1,B2[J]. FOOD SCIENCE, 2004, 25(6): 153-156.

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Comparison in Determination Methods of Vitamin B1,B2

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