FOOD SCIENCE ›› 2004, Vol. 25 ›› Issue (9): 166-169.

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Protective Effects of Tea Polyphenols on Ni2O3-induced Lipid Peroxidation in Mice Alveolar Macrophage and DNA Strand Breakage of Human Lung Fibroblasts Cell

 ZHANG  Jing, ZHANG  Jun, SHI  Hong-Jun, LI  Zhuo-Quan, GUO  Chao, GUO  Jun-Sheng   

  1. 1.College of Life Science and Technology Tongji University;2.Department ofMilitary Hygiene, Faculty of Navy Medicine, Second Military Medical University;3. College of Medical Science, Biological Teaching Research Room, Tongji University
  • Online:2004-09-15 Published:2011-10-24

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Abstract: To study the effects of tea polyphenols (TP) on the injury of mice alveolar macrophages treated with Ni2O3, the alveolarmacrophages were cultured in vitro with exposure to Ni2O3(5mg/L). Meanwhile, TP(125, 250 and 500mg/L)were added in themedium, respectively. The detection of malondialdehyde and the activity of antioxidant enzyme in alveolar macrophages werecarried out. The protective effects of TP against DNA damage of human lung fibroblasts (HLF) cells induced by Ni2O3 were alsoinvestigated. HLF cells were cultured in vitro and treated with Ni2O3(5mg/L) and Ni2O3+TP(250mg/L), respectively. The singlecell gel electrophoresis (SCGE) was used to detect the DNA breaks. The results showed that TP could increase the activity ofsuperoxide dismutase in mice alveolar macrophages in a dose-dependent manner and inhibit significantly the production ofmalondialdehyde at dose of 250mg /L and 500mg/L respectively in comparison with Ni2O3 group(p<0.05). The level of DNAbreaks induced by Ni2O3 was higher than that of the control group (p<0.01). But the level of DNA breaks induced by Ni2O3+TP was lower than that of the Ni2O3 treated group (p<0.01). All results demonstrated that TP could prevent cells from DNAdamage induced by Ni2O3.

Key words: tea polyphenols, Ni2O3, MDA, SOD, DNA damage, SCGE