FOOD SCIENCE ›› 2004, Vol. 25 ›› Issue (10): 119-122.

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Extraction, Separation and Determination of Polysaccharides and Testing of Selenium(Se) in Polygonatum

 WANG  Xing-Ping, MO  Kai-Ju, ZHOU  Da-Zhai, DAN  Chang-Hai   

  1. School of Biological Science and Technology, Hubei Institute for Nationalities
  • Online:2004-10-15 Published:2011-10-24

Abstract: This article used the medicinal material-Polygonatum which was rich in senelium element as experimental material,preliminarily studied the separating and extracting technology of Senelium polysaccharides.The experiments studied the extract-ing technology by the methods of water extracting, preciptation with alcohol, sevage deproteinizing, preciptation,vacuum freezingdrying and the content of polysaccharides and senelium were determined by anthrone colorimetry and atomic absorptionspectrometry.The optimum parameters to extract soluble selenium polysaccarides in Rhizoma Polygonati were as follows:temperature 90℃, extracting time 4h, the ratio of water to material 1:50, extracting times 2,the content of soluble seleniumpolysaccarides 3.12%.

Key words: polygonatum polysaccharides, extraction, selenium(Se)