FOOD SCIENCE ›› 2008, Vol. 29 ›› Issue (10): 148-152.

Previous Articles     Next Articles

Study on Extraction Technology of Flavonoids from Rape Bee Pollen

 LU  Ting, GUO  Xue-Wei, MA  Lan-Yu, WU  Ling-Ge   

  1. 1.Qinghai Institute of Supervision and Inspection on Product Quality,Xining 810008,China; 2.Daxing People’s Hospital,Beijing 102600,China
  • Online:2008-10-15 Published:2011-12-08

Abstract: Technological extraction of flavonoids in rape bee pollen was studied by using the methods of ethyl alcohol extraction and water precipitation combined with macroporous resin concentration and ethyl alcohol elution,and the satisfying results were obtorined. The transfer rate of flavonoids in rape bee pollen is over 70%,and the content of flavonoids is over 50%. Rutin,quercetin,kaempferol and isorhamnetin are separated from extracts by the methods of column separation and stepping elution of organic solvent. Rutin,quercetin and kaempferol are verified by appraisal of nuclear magnetic resonance. Meanwhile,β-sitosterol and β-stigmasterol are separated by the methods of concentration and elution. The bioactive substances are verified by nuclear magnetic resonance.

Key words: bee pollen, flawonoids, extraction