Abstract: Under the fixed conditions of 10% substrate,Flavourzyme -Papain ratio 1:1,the effects of pH,enzyme amount,temperature,reaction time on hydrolysis degree of Procambarus clarkii byproduct were investigated,and these factors were optimized by orthogonal test. The optimal substrate concentration was also determined. Results showed that the optimal hydrdysis conditions are,pH 7.0,amount of compound enzyme1.5%,temperature 50～55℃,time 5 hours and substrate concentration 25%. The maximum hydrolysis degree reaches 26.2%.

Key words: Flavourzyme-Papain compound enzyme, Procambarus clarkii byproduct, hydrolysis degree