Abstract: This study aimed to evaluate the applicability of BacT/ALERT 3D system used in detection of commercial sterilization of konjac cans. 59 samples of konjac cans were incubated according to the method with BacT/ALERT 3D system and GB/T4789.26 method. 19 positive samples detected by BacT/ALERT 3D system were tested and analyzed by Japanese test method and GB/T4789.26 method. The results showed that BacT/ALERT 3D system combined with validity test by Japanese method can rapidly and truly detect microorganism in konjac cans,and there are higher false negative rates when the konjac cans with high pH value are detected for commercial sterilization by GB/T4789.26 method.

Key words: BacT/ALERT 3D system, konjac can, commercial sterilization