Study on ELISA Detection Method of Hepatitis A Virus in Strawberry

Abstract

Abstract: Monoclonal antibody against hepatitis A virsus (HAV) was labeled with horseradish peroxides (HRP) in glutaraldehyde,and a double-antibody sandwich ELISA (DAS-ELISA)method for detection of the HAV was developed. The optimal working concentration for HRP-antibody at a dilution of 1:100 is determined,and the lowest detection amount of the HAV dissolved in PBS is determined as 2μg/ml. When adding the inactivated HAV to strawberry juice,the detection limit of the antigen is 4μg/ml by ELISA. The ELISA is convenient,reliable and suitable method for detection of HAV in strawberries.

Key words: ELISA, hepatitis A virus (HAV), strawberry, detection

MA Ji-Xiang, TIAN Shi-Min, ZOU Ming-Qiang, JIN Yong, CHEN Yan-Chang, XUE Qiang. Study on ELISA Detection Method of Hepatitis A Virus in Strawberry[J]. FOOD SCIENCE, 2008, 29(10): 459-462.

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Study on ELISA Detection Method of Hepatitis A Virus in Strawberry

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