Study on Enzymatic Preparation of Quercetin from Crude Alcohol Extract of Vaccininm uliginosum L.

Abstract

Abstract: Using orthogonal test design,the parameters for extracting quercetin from crude alcohol extract of Vaccininm uliginosum L. were optimized. The content of quercetin was determined by HPLC in order to investigate the extraction processing. The effect extent of the main factors ranks as:sulfuric acid concentration >hydrolyze time > sulfuric acid amount. The optimal extraction process is adding the crude alcohol extract into 60 times of 2% sulfuric acid,and then boiling for 1 h for hydrolysis. By this process,the quercetin concent in solution is increased from 0.398% to 1.984%.

Key words: Vaccininm uliginosum L., rutin, hydrolysis, quercetin

LU Yue, LIN Song-Yi, ZHANG Yan, LIU Jing-Bo. Study on Enzymatic Preparation of Quercetin from Crude Alcohol Extract of Vaccininm uliginosum L.[J]. FOOD SCIENCE, 2008, 29(12): 323-326.

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Study on Enzymatic Preparation of Quercetin from Crude Alcohol Extract of Vaccininm uliginosum L.

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