FOOD SCIENCE ›› 2008, Vol. 29 ›› Issue (12): 385-388.

Previous Articles     Next Articles

Purification of Carp Parvalbumin Allergen

 XIAO  You-Ming, LIU  Hong, CHEN  Hong-Bing, GAO  Jin-Yan   

  1. 1.State Key Laboratory of Food Science and Technology,Nanchang University,Nanchang 330047,China; 2.Department of Food Science,Nanchang University,Nanchang 330047,China; 3.Department of Animal Science,Jiangxi Biological Science and Technology Vocational College,Nanchang 330200,China;4. Sino-German Joint Research Institute,Nanchang University,Nanchang 330047,China
  • Online:2008-12-15 Published:2011-12-08

Abstract: Carp parvalbumin is one of important allergens in carp. The purified carp parvalbumin was botained by anion-exchange chromatography on DEAE-Sepharose fast fow . The results showed that the purity of the carp parvalbumin is more than 90 percent,which was identified with SDS-PAGE and PAGE. This method can provide a useful way to purify carp parvalbumin allergen.

Key words: carp, parvalbumin, anion-exchange chromatography, purification, allergen