Abstract: This article studied the combined forms and distribution of selenium, the extraction and separation technology of macromolecule containing selenium in pumpkin. Uses the distilled water, the thin salt, the ethyl alcohol, the thin alkali to extract separately in turn, the polysaccharide was selected with hot water immersion extraction. After that, selenium was assayed with the atomic absorption spectrophotometric method. The results indicated that, in the pumpkin, the main Se-containing components are Se-protein and Se-polysaccharide, occupying the total the selenium 45.76% and 27.47%. In Se-protein components, alkali- soluble protein has the highest selenium content, which occupies the total selenium 46.34%. Thus, the Enshi rich selenium pumpkin is extremely good function food or the drugs, having huge developmental potential.

Key words:  , pumpkin； Se-protein； Se-polysaccharide； combined forms；