Abstract: The cordycepin in culture medium of the cordyceps militaris link were extracted by microwave treatment. HPLC was used to determine the content of cordycepin in extracting solution of cordyceps militaris link. And the orthogonal test design L9 (34) was used to optimize the conditions of microwave treatment. The optimum conditions of microwave treatment were as follows: extracting solution volume 10ml, solid-liquid ratio 1:200, middle firepower treating (microwave irradiation power 50W) for 3min. Content of the cordycepin in sample was 12.16mg/g .The extraction time of microwave was 1/40 of supersonic extraction and extraction rate of microwave is higher than supersonic.

Key words:  , microwave extraction； cordycepin； orthogonal test design； HPLC；