Abstract: Food contaminated with bacterial pathogens are major cause of food-borne disease. Rapid detection of food-borne bacteria is essential to efficiently prevent pathogens prevalence and food poisoning. Multiplex PCR which can simultaneously detect more than one bacteria has been applied increasingly and widely. This paper has introduced the application of multiplex PCR for detection of food-borne bacterial pathogens and the optimization of multiplex PCR reaction conditions. Moreover some problems and perspectives have been brought up.

Key words:  , multiplex PCR (polymerase chain reaction)； bacterial pathogen； detection；