Primary Study on Separation and Purification of a Microbial Transglutaminase

Abstract

Abstract: The enzyme of microbial transglutaminase (MTG) can modify the structure and properties of proteins through catalyzing an acyl transfer reaction of a γ-carboxyamide group of glutamine residue in a peptide chain, resulting the cross-linking of proteins. In this paper, based on the general physical and chemical character of MTG, several satisfied methods was decided after studied on using high centrifugation, ammonium sulfate precipitation, alcohol precipitation, acetone precipitation to separation MTG.

Key words: , microbial； transglutaminase； separation； precipitation； enzymatic character；

HAO Wei, WANG Zhang, CAI Hui-Nong. Primary Study on Separation and Purification of a Microbial Transglutaminase[J]. FOOD SCIENCE, 2006, 27(10): 331-334.

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Primary Study on Separation and Purification of a Microbial Transglutaminase

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