Synthesis and Identification for Salbutamol Antificial Antigen

Abstract

Abstract: Salbutamol is actived by succinic anhydride and converted into salbutamol-succinate as the hapten. The results of IR, 1H-NMR and MS indicated that the hapten was synthesized successfully and identified as an inner salt. Salbutamol-succinate was linked to carrier protein bovine serum albumin (BSA) as immunogen, and ovalbumin(OVA) as coating antigen by mixed anhydride method and NHS ester method respectively. Antigens SAL-BSA and SAL-OVA were prepared successfully and confirmed by ultraviolet spectrum. The conjugation ratio of SAL-BSA and SAL-OVA were 12.8:1, 23:1, respectively.

Key words: salbutamol, hapten, identification

YUAN Li-peng,SUN Yuan-ming,LEI Hong-tao,SHEN Yu-dong, HUANG Xiao-yu,PAN Ke,HUANG Jie. Synthesis and Identification for Salbutamol Antificial Antigen[J]. FOOD SCIENCE, 2006, 27(12): 276-280.

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Synthesis and Identification for Salbutamol Antificial Antigen

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