Abstract: To improve the purification and function of oat β-glucan such different ways as the way of Sevag, using trypsinase, precipitating protein at pH4.5, using trypsinase before precipitating protein at pH4.5 and the way of Sevag together with trypsinase were used. The results showed the Ratio of removing protein didn’t change any longer after using the way of Sevag six times if experiment was added, time of experiment decreased by the way of Sevag together with trypsinase, ratio of removing protein using trypsinase was not high, the operation was simple and cost of experiment was low when protein was precipitated at pH4.5, and after using trypsinase result of removing protein at pH4.5 was the best among them. Ratio of removing protein was 95.8%, which ratio of keeping polysaccharide was 90.6%.

Key words: liquid of extracting polysaccharide, removing protein, compare