Abstract: Grifola frondosus is an edible and medicinal mushroom with rich polysaccharides ,amino acids and mineral elements. It is a new effective way to supply zinc to human body that Grifola frondosus is used to transfer inorganic zinc to organic zinc what can be easily absorbed by human body. The rare earth element lanthanide has effect of promoting on the growth of Grifola frondosus. For studying the ability of Grifola frondosus to accumulate zinc when cultured in liquid with the rare earth element lanthanide, authors designed a orthogonal test schemes which contained 4 factors and 4 levels. The factors and levels were as follows: La(NO3)3 concentration was 50, 100, 150, 200(mg/L culture medium); potato/glucose 150/18, 200/24, 250/30, 300/36 (g/g, 1L culture medium); bran 20, 25, 30, 35(g/L); ZnSO·47H2O concentration 100, 600, 1300, 2000(mg/L culture medium). The authors have used four criteria to analyze the experiments results and have obtained the optimum culture conditions for zinc amount in dry biomass, organic zinc yield and the zinc translation rate in order to guide technology design. With organic zinc yield in 25ml culture medium as criterion, the 1L optimal culture medium consists of ZnSO·47H2O 2000mg, bran 35g, La(NO3)3 50mg, potato 300g, glucose 36g. The organic zinc yield in 25ml culture medium is 1.472mg/25ml, that is 58.88mg/L culture medium.

Key words: Grifola frondosus, liquid culture, Zinc, lanthanide