FOOD SCIENCE ›› 2006, Vol. 27 ›› Issue (12): 501-504.

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Study on Preparation for Oligo-peptides of Branch-chain Amino Acids from Konjac Fly Powder by Solidified Kefir Mixed Culture

 HUANG  Hao, ZHAO  Shan-Shan, GAN  Xin   

  1. 1.College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430068, China; 2.College of Bioengineering, Hubei University of Technology, Wuhan 430064, China
  • Online:2006-12-15 Published:2011-11-23

Abstract:  We get the optimum technology of fermentation on the target of transform rate of starch and protein in konjac powder around fermentation is that at 35℃, we culture it on table (130r/min) for 36h.Molecular of peptide is about 10000D that is determined by SDS-PAGE gel-electrophoresis. We get oligo-peptides of branch chain amino acids from the peptides by two process enzymatic hydrolysis. The oligo-peptides can be separated by gelatin and analyzed by automatic analytical balance. The conclusion is: The content of branch chain amino acids in oligo-peptides is 27.52%, having reached the most favorable condition (25%~65%).

Key words:  , kefir; konjac flying powder; high content branch chain amino acids;